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KMID : 0923620090090060274
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Immune Network
2009 Volume.9 No. 6 p.274 ~ p.284
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Swiprosin-1 Regulates Cytokine Expression of Human Mast Cell Line HMC-1 through Actin Remodeling
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T.P. Ramesh
Kim Young-Dae
Kwon Min-Sung
Jun Chang-Duk
Kim Sang-Wook
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Abstract
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Background: Swiprosin-1 was identified in human CD8＋ lymphocytes, mature B cells and non-lymphonoid tissue. We have recently reported that swiprosin-1 is expressed in mast cells and up-regulated in both in vitro and in vivo.
Methods: The expression of cytokines and swiprosin-1 were determined by by real time PCR and conventional PCR. Pharmacological inhibitors were treated to investigate potential mechanism of swiprosin-1 in mast cell activation. Actin content was evaluated by confocal microscopy and flow cytometry.
Results: The swiprosin-1 augmented PMA/A23187- induced expression of cytokines and release of histamine. However, knock-down of swiprosin-1 showed only a modest effect on PMA/A23187-induced cytokine expression, suggesting that swiprosin-1 has gain-of-function characteristics. Swiprosin-1 was found in microvilli-like membrane protrusions and highly co-localized with F-actin. Importantly, either disruption of actin by cytochalasin B or inhibition of PI3 kinase, an enzyme involved in actin remodeling, by wortmannin blocked cytokine expression only in swiprosin-1-overexpressing cells.
Conclusion: These results suggest that swiprosin-1 modulates mast cell activation potentially through actin regulation.
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KEYWORD
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Mast cells, Swiprosin-1, Protein kinase C, Actin remodeling
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